| 货号 | 61325 |
| 克隆类别 | HTA28 |
| 同种亚型 | IgG2a |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Rat |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Ascites |
| 免疫原 | This Histone H3 phospho Ser28 antibody was raised in rat against a peptide corresponding to amino acid residues 23-35 (phospho Ser 28) of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | This Histone H3 phospho Ser28 antibody is provided as ascites fluid. For your convenience, an IgG versi |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 phospho Ser28 antibody tested by Western blot. 5 µg HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) was run on a 12% PAGE gel, transferred to a PVDF membrane and probed with Histone H3 phospho Ser28 antibody at a 1:1,000 dilution. |
