| 货号 | 39681 |
| 克隆类别 | MABI 0306 |
| 同种亚型 | IgG2a |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 0.5 - 2 µg/ml dilution Published Applications: ChIP See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Histone H3 monomethyl Lys9 antibody was raised against a synthetic peptide containing monomethyl Lys9 of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a PBS only version of this antibody is also available. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 monomethyl Lys9 antibody tested by Western blot. HeLa nuclear extract (20 µg) probed with Histone H3 monomethyl Lys9 antibody (2 µg/ml dilution). | |
Histone H3 monomethyl Lys9 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 monomethyl Lys9 antibody was applied at a dilution of 1:2,000 to Active Motifs MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the H3 monomethyl Lys9 peptide is inhibited by a citrulline at position 8 and blocked by Ser10 and Thr11 phosphorylation. Array Data File | |
Histone H3 monomethyl Lys9 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys9 mAb for monomethyl-Lys9 histone H3. Methylated recombinant histone proteins corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 0.3 µg/ml. The amount of protein (picomoles) spotted is indicated next to each row. Lane 1: monomethyl lysine 9 protein. Lane 2: dimethyl lysine 9 protein. Lane 3: trimethyl lysine 9 protein. No detection of proteins (unmodified, mono-, di-, or tri-methylated) corresponding to lysine 27 of histone H3 was observed with Histone H3 monomethyl Lys9 antibody. |
