| 货号 | 39539 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution Published Applications: see references This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39173. |
| 供应商 | Active Motif |
| 纯化方式 | Culture Supernatant |
| 免疫原 | This Histone H4 dimethyl Lys20 antibody was raised against a peptide including dimethyl-Lys20 of human histone H4. |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 8 kDa |
| 参考文献 |
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Histone H4 dimethyl Lys20 mAb tested by Western blot. Detection of dimethylated Histone H4 by Western blot. The analysis was performed using HeLa acid extract (10 µg, lane 1) and recombinant histone H4 protein (200 ng, lane 2) probed with Histone H4 dimethyl Lys20 mAb at a 1:2,000 dilution. |
