| 货号 | 39536 |
| 克隆类别 | 7B11 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 0.5 - 2 µg/ml dilution WB: 0.5 - 2 µg/ml dilution Published Applications: see references This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39535. |
| 供应商 | Active Motif | |
| 纯化方式 | Protein A Chromatography | |
| 免疫原 | This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3. | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or H3K27me3) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Purified IgG in 10 mM sodium phosphate (pH 7.5), 150 mM NaCl, 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 di/trimethyl Lys27 antibody (mAb) tested by immunofluorescence. Top: HeLa cells stained with Histone H3 di/trimethyl Lys27 antibody (mAb) at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain™ Immunofluorescence Tools. | |
Histone H3 di/trimethyl Lys27 antibody (mAb) tested by Western blot. HeLa acid extract (10 µg) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500). | |
Histone H3 di/trimethyl Lys27 antibody (mAb) specificity data. HeLa acid extract (10 µg per lane) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500) with or without pre-incubation of antibody with 1 µM peptide containing the sequence surrounding either Lys9 or Lys27 of Histone H3. Lane 1: no peptide Lane 2: unmodified Lys27 Lane 3: monomethyl-Lys27 Lane 4: dimethyl Lys27 Lane 5: trimethyl-Lys27 Lane 6: unmodified Lys9 Lane 7: monomethyl-Lys9 Lane 8: dimethyl-Lys9 Lane 9: trimethyl-Lys9. |
