| 货号 | 39285 |
| 克隆类别 | 2AG-6F12-H4 |
| 同种亚型 | IgG1k |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:20 - 1:100 dilution WB: 1:250 - 1:1,000 dilution Published Applications: see references This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39161. |
| 供应商 | Active Motif |
| 纯化方式 | Culture Supernatant |
| 免疫原 | This Histone H3 trimethyl Lys9 antibody was raised against a peptide containing trimethyl-lysine 9 of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Concentrated culture supernatant containing 30% glycerol, 0.035% sodium azide. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by immunofluorescence. Staining of HeLa cells with Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) (1:250 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel). | |
Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by Western blot. HeLa acid extract (10 µg per lane) probed with Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) (1:500 dilution). | |
Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys9 mAb (Clone 2AG-6F12-H4) for trimethyl Lys9 of histone H3. Various methylated H3 proteins were spotted onto PVDF and probed with 39285 at 1:750. The amount of protein (picomoles) spotted is indicated next to each row. Lane 1: monomethyl-Lys9 modified-lysine analog of histone H3. Lane 2: dimethyl-Lys9 modified-lysine analog of histone H3. Lane 3: trimethyl Lys9 modified-lysine analog of histone H3. |
