| 货号 | 61479 |
| 克隆类别 | A1 |
| 同种亚型 | IgG1 |
| 反应种属 | Other (Not Species Specific) |
| 来源宿主 | Mouse |
| 应用 | Methyl-DNA Immunoprecipitation/Dot Blot |
| 使用方法 | Validated Applications: MeDIP: 1 - 2 µg per IP MeDIP-Seq: 1 - 2 µg each DB: 0.5 - 2 µg/ml dilution In MeDIP, the DNA must be denatured when using this antibody. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine. |
| 背景 | 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody(Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 参考文献 |
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Next-Gen sequencing data generated using 5-Methylcytosine (5-mC) antibody (mAb) correlates well with CpG density. DNA was enriched from 1 ug of denatured, adaptor ligated human PC9 cell DNA using 2 ul of the 5-Methylcytosine (5-mC) antibody (mAb). MeDIP DNA was sequenced using the Illumina platform to generate 26 million sequence tags. Tags were mapped to generate a whole-genome DNA methylation profile. The image above shows that the enriched regions (purple peaks) correlate well with CpG density (green overlay). | |
5-Methylcytosine (5-mC) antibody (mAb) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with MseI was subjected to MeDIP using 2 µg of the 5-Methylcytosine antibody and then analyzed by quantitative real-time PCR with primers specific for ZC3H13 that is normally methylated or Untr1 that is normally unmethylated. The MeDIPd DNA (enriched DNA) was plotted. | |
5-Methylcytosine (5-mC) antibody (mAb) tested by dot blot analysis. DNA from the Methylated DNA Standard Kit (Catalog No. 55008) were spotted (5 ng per spot) on to a positively charged nylon membrane and blotted with 5-Methylcytosine antibody (2 µg/ml dilution). Lane 1: single-stranded unmethylated DNA. Lane 2: single-stranded DNA containing 5-methylcytosine. Lane 3: single-stranded DNA containing 5-hydroxymethylcytosine. Lane 4: double-stranded unmethylated DNA. Lane 5: double-stranded DNA containing 5-methylcytosine. Lane 6: double-stranded DNA 5-hydroxymethylcytosine. |
