| 货号 | 39216 |
| 克隆类别 | 106B691 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This MBD3 antibody was raised against a synthetic peptide corresponding to human MBD3. |
| 背景 | MBD3(methyl-CpG binding domain protein 3) is a subunit of the NuRD, a multi-subunit complex containing nucleosome remodeling and histone deacetylase activities. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD3 regulates transcription by forming a Mi-2/NuRD complex with nucleosome remodeling and histone deacetylase (HDAC) activities in mammalian cells. MBD3 has been shown to interact not with 5-methylcytidine methylated DNA but with DNA containing 5-hydroxymethylcytosine (5-hmC) methylation. MDB3 co-localizes with TET1 and is required for normal levels of 5-hmC in embryonic stem cells. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 50 kDa |
| 参考文献 |
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MBD3 mAb tested by Western blot. The analysis was performed using HeLa whole-cell extract and MBD3 mAb at 2 µg/ml (lane 1) and a 1 µg/ml (lane 2) dilutions. |
