| 货号 | 39206 |
| 克隆类别 | 64B1446 |
| 别名 | DNMT3A2, DNA Methyltransferase 3a |
| 同种亚型 | IgG1k |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: WB: 2 - 4 µg/ml dilution Published Applications: ChIP See references for more information. Individual optimization may be required. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This DNMT3A antibody was raised against a full-length protein corresponding to mouse DNMT3A. |
| 背景 | DNMT3A–DNA methyltransferase proteins such as DNMT3A (or DNMT 3A) are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases,DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.05% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 116 kDa |
| 参考文献 |
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DNMT3A mAb tested by Western blot. Detection of DNMT3A by Western blot. The analysis was performed using DNMT3A mAb at a 2 µg/ml dilution and whole-cell extracts from 293 cells that had been transfected with plasmid expressing either mouse DNMT3A (lane A) or DNMT3B (lane B). |
