| 货号 | 39649 |
| 克隆类别 | 33D3 |
| 同种亚型 | IgG |
| 反应种属 | Other (Not Species Specific) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Methyl-DNA Immunoprecipitation/Immunofluorescence/Dot Blot/Immunohistochemistry/Enzyme-linked Immunosorbent Assay/Flow Cytometry/Immunocytochemistry |
| 使用方法 | Validated Applications: MeDIP: 1 µg per IP ICC/IF: 0.5-5 µg dilution WB: 1:250 dilution ELISA: 1:10,000 dilution Published Applications: MeDIP ICC/IF DB See references for more information. Individual optimization may be required. Working dilutions of IHC&FACS will be assay dependent. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA. |
| 背景 | 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody(Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 10 mM phosphate, 0.15 M NaCl and 0.01% thimerosal. Thimerosal is highly toxic. |
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5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIPd DNA (enriched DNA) was plotted. |
