| 货号 | 61689 |
| 别名 | ARF, MLM, P14, P19, CMM2, INK4, MTS1, CDK4I, CDKN2, MTS-1, P14ARF, P19ARF |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the N-terminal region of human CDKN2A p14ARF. |
| 背景 | CDKN2A p14ARF (Cyclin-Dependent Kinase Inhibitor 2A) is capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its Lys63-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP. There are several transcripts encoding functionally different proteins from the CDKN2A gene. p16 INK4a, p16 gamma and p12 use the first exons to generate structurally related protein isoforms and function as CDK4 inhibitors. p14ARF is also encoded by the same CDKN2A gene but utilizes an alternative 1st exon which generates a protein that is structurally unrelated to the other proteins. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 18 kDa |
| 参考文献 |
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CDKN2A p14ARF antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (Lane 1, 25 µg) and nuclear extract of Saos cells (Lane 2, 40 µg) probed with CDKN2A p14ARF antibody (pAb) at a dilution of 1:500. |
