| 货号 | 61639 |
| 别名 | PARP, PPOL, ADPRT, ARTD1, ADPRT1, ADPRT 1, pADPRT-1 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µg per IP WB: 0.2 - 1 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a His-tagged fusion protein corresponding to the N-terminal half of human PARP-1. |
| 背景 | PARP-1 N-terminal(ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1(ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version ( |
| 计算分子量 | 120 kDa |
| 参考文献 |
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PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation. 10 µg of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 µg/ml. | |
PARP-1 N-terminal antibody (pAb) tested by Western blot. HeLa nuclear extract (25 µg) was probed with PARP-1 N-terminal antibody at a dilution of 1 µg/ml. |
