| 货号 | 61607 |
| 别名 | AOF2, KDM1, LSD1, BHC110 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a recombinant protein corresponding to human KDM1A. |
| 背景 | KDM1A (Lysine (K)-Specific Demethylase 1A) was identified as a subunit of different complexes (such as CtBP, CoREST, NuRD or BRAF35). KDM1A is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. KDM1A is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. KDM1A is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. KDM1A is a flavin-dependent amine oxidase. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 110 kDa |
| 参考文献 |
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KDM1A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 20 µg of chromatin from K562 cells and 10 µl KDM1A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
KDM1A antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (30 μg) probed with KDM1A antibody at a dilution of 1:500. |
