| 货号 | 61557 |
| 别名 | RPB2, POL2RB, hsRPB2, hRPB140 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 5 - 10 µl per IP WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the N-terminal region of human POLR2B. |
| 背景 | POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity). |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 135 kDa |
| 参考文献 |
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POLR2B antibody (pAb) tested by Immunoprecipitation. 10 µl of POLR2B antibody was used to immunoprecipitate POLR2B from 200 µg of HEK293 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the POLR2B antibody at a dilution of 1:500. | |
POLR2B antibody (pAb) tested by Western blot. Nuclear extract of HEK293 cells (30 µg) probed with POLR2B antibody (pAb) at a dilution of 1:1,500. |
