| 货号 | 61369 |
| 别名 | ANCCA, CT137, PRO2000 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 5 - 10 µl per IP WB: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the C-terminal region of human ATAD2. |
| 背景 | ATAD2 (ATPase family AAA domain-containing protein 2) may be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 160 kDa |
| 参考文献 |
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ATAD2 antibody (pAb) tested by Immunoprecipitation. 10 µl of ATAD2 antibody was used to immunoprecipitate ATAD2 from 250 µg of Jurkat nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ATAD2 antibody at a dilution of 1:1,000. | |
ATAD2 antibody (pAb) tested by Western blot. ATAD2 detection by Western blot. The analysis was performed using 40 µg Jurkat nuclear cell extract and CENP-B at a 1:1,000 dilution. |
