| 货号 | 61307 |
| 别名 | RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This RNA Pol II CTD phospho Thr4 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at threonine 4. |
| 背景 | RNA pol II(RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair.RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 240 kDa |
| 参考文献 |
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RNA pol II CTD phospho Thr4 antibody pAb tested by Western blot. Nuclear extract of HeLa cells (40 µg) probed with RNA pol II CTD phospho Thr4 antibody at a dilution of 2 µg/ml. | |
RNA Pol II CTD phospho Thr4 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of RNA Pol II CTD phospho Thr4 pAb. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho Thr4 of RNA Pol II CTD peptide. Lane 2: Unmodified Thr4 of RNA Pol II CTD peptide. Lane 3: Phospho Ser2 of RNA Pol II CTD peptide. Lane 4: Phospho Ser5 of RNA Pol II CTD peptide. |
