| 货号 | 61301 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µl per IP WB: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Elk-1 antibody was raised against a peptide within the C-terminal region of human Elk-1. |
| 背景 | Elk-1 (ETS-like gene 1) is a member of the ETS family of transcription factors and stimulates transcription. It binds to purine-rich DNA sequences, and can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 68 kDa |
| 参考文献 |
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Elk-1 antibody (pAb) tested by Immunoprecipitation. 10 µl of Elk-1 antibody was used to immunoprecipitate Elk-1 from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Elk-1 antibody at a dilution of 1:500. | |
Elk-1 antibody (pAb) tested by Western blot. Whole cell extract of HeLa cells (40 µg) probed with Elk-1 antibody (1:500). |
