| 货号 | 61247 |
| 别名 | Non-POU Domain Containing, Octamer-Binding, 54 KDa Nuclear RNA- And DNA-Binding Protein, P54, NMT55, NRB54, P54NRB |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 5 µg per IP WB: 0.5 - 2 µg/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This NonO antibody was raised against a peptide corresponding to the C-terminus of human NonO. |
| 背景 | NonO (non-POU domain containing, octamer-binding, p54(nrb), NRB54, NMT55) is a DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. This gene also encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 60kD |
| 参考文献 |
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NonO antibody (pAb) tested by Immunoprecipitation. 5 µg of NonO antibody was used to immunoprecipitate NonO from 400 µg of HeLa whole cell extract (lane 2). 5 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the NonO antibody at a dilution of 1 µg/ml. | |
NonO antibody (pAb) tested by Western blot. Whole cell extract of HeLa cells (20 µg) probed with NonO antibody (1 µg/ml dilution). |
