| 货号 | 61221 |
| 别名 | TDRD14B |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:2,000 dilution IP: 10 µl per IP WB: 1:500 - 1:1,000 dilution For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This JMJD2B / KDM4B antibody was raised against a peptide within the C-terminal region of human JMJD2B. |
| 背景 | JMJD2B / KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B / KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERα-mediated transcriptional activation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 142 kDa |
| 参考文献 |
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JMJD2B / KDM4B antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with JMJD2B / KDM4B antibody at a 1:2,000 dilution. | |
JMJD2B / KDM4B antibody (pAb) tested by Immunoprecipitation. 10 µl of JMJD2B / KDM4B antibody was used to immunoprecipitate JMJD2B / KDM4B from 500 µg of K562 whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JMJD2B / KDM4B antibody at a dilution of 1:500. | |
JMJD2B / KDM4B antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (40 µg) probed with JMJD2B antibody (pAb) at a dilution of 1:500. |
