货号 | 61217 |
别名 | PHB1, HEL-215, HEL-S-54e |
同种亚型 | IgG |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | Western Blot |
使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
供应商 | Active Motif |
纯化方式 | Affinity Purified |
免疫原 | This PHB / prohibitin antibody was raised against a peptide within the N-terminal region of human PHB. |
背景 | PHB / Prohibitin is a key cell cycle regulatory protein, inhibiting cell division by inactivating E2F family proteins, and has potent anti-proliferative activity. PHB / Prohibitin is also involved in repression of transcription through androgen receptor. |
运输条件 | Blue Ice |
存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
计算分子量 | 30 kDa |
参考文献 |
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PHB / Prohibitin antibody (pAb) tested by Western blot. Whole-cell extract of MCF-7 cells (25 µg) probed with PHB antibody (pAb) at a dilution of 1:500. |