| 货号 | 61137 |
| 别名 | STAT5 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µg per IP WB: 0.5 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This STAT5B antibody was raised against a peptide in the C-terminal region of human STAT5B. |
| 背景 | STAT5AandSTAT5B are encoded by separate genes but are 90% identical. They mediate gene expression in response to IL-2, IL-3, IL-7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. The STAT5A/B signaling pathway is involved in the transition of organ-confined prostate cancer to hormone-refractory disease. This implies that the active JAK2-STAT5A/B signaling pathway potentially provides the means for pharmacological intervention of clinical prostate cancer progression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 90 kDa |
| 参考文献 |
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STAT5B antibody (pAb) tested by Immunoprecipitation. 10 µg of STAT5B antibody was used to immunoprecipitate STAT5B from 400 µg of K562 cytosolic cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the STAT5B antibody at a dilution of 1 µg/ml. | |
STAT5B antibody (pAb) tested by Western blot. STAT5B antibody detection by Western blot. The analysis was performed using K-562 cytosolic-cell extract (25 µg) and STAT5B antibody (pAb) at a 1 µg/ml dilution. |
