| 货号 | 61109 |
| 别名 | CDR, MTG8, AML1T1, ZMYND2, CBFA2T1 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µl per IP WB: 1:500 - 1:2,000 dilution For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This ETO / RUNX1T1 antibody was raised against a peptide within the internal region of human ETO / RUNX1T1. |
| 背景 | ETO / RUNX1T1 (AML1T1, CBFA2T1, MTG8, ZMYND2) belongs to a family of evolutionarily conserved nuclear factors that act as a transcriptional corepressor. ETO / RUNX1T1 does not interact directly with DNA but is recruited by transcription factors such as PLZF, BCL6, TAL1/SCL, Gfi1and Heb recruit nuclear co-repressors such as NCoR, SIN3, and SMRT / NCoR2, which in turn interact with histone deacetylase (HDAC) to mediate transcriptional repression. ETO / RUNX1T1 contains four evolutionarily conserved functional domains called nervy homology regions (NHRs) involved in protein-protein interaction. ETO / RUNX1T1 is frequently found as a fusion partner of AML1 in acute myeloid leukemia with the t(8;21) translocation which gives rise to the AML-ETO fusion protein. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 68 kDa |
| 参考文献 |
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ETO / RUNX1T1 antibody (pAb) tested by Immunoprecipitation. 10 µl of ETO / RUNX1T1 antibody was used to immunoprecipitate ETO / RUNX1T1 from 250 µg of K562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ETO / RUNX1T1 antibody at a dilution of 1:500. |
