| 货号 | 61069 |
| 别名 | DRIP5 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µl per IP WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This RAP1 / TERF2IP antibody was raised against a peptide within human RAP1 / TERF2IP. |
| 背景 | RAP1/TERF2IP(Repressor/activator protein 1,Telomeric repeat-binding factor 2-interacting protein 1) is a protein associated with telomeres that acts both as a modulator of telomere function and as a regulator of transcription. RAP1 is a component of the shelterin (telosome) protein complex and is involved in the regulation of telomere length and serves to protect telomeric repeats from degradation. In contrast to other components of the shelterin complex, RAP1 is dispensable for telomere end-capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. RAP1 is recruited to telomeric repeats via its interaction with TERF2. Independent of its function in telomeres, RAP1 also acts as a transcriptional regulator. It is recruited to non-telomeric 5-TTAGGG-3 sites via its association with TERF2 or other factors, and regulates gene expression. RAP1 is also present in the cytoplasm and associates with the IκB-kinase (IKK) complex and acts as a regulator of the NFκB signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activation of the expression of NFκB target genes. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 55 kDa |
| 参考文献 |
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RAP1 / TERF2IP antibody (pAb) tested by Immunoprecipitation. 10 µl of RAP1 / TERF2IP antibody was used to immunoprecipitate RAP1 / TERF2IP from 250 µg of K-562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the RAP1 / TERF2IP antibody at a dilution of 1:500. | |
RAP1 / TERF2IP antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (30 µg) probed with RAP1 / TERF2IP antibody (pAb) at a dilution of 1:500. |
