| 货号 | 40970 |
| 别名 | HD5, NY-CO-9 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution Published Applications: ChIP See references for more information. Individual optimization may be required. For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This HDAC5 antibody was raised against a synthetic peptide corresponding to amino acid residues 572-587 of human HDAC5. |
| 背景 | HDAC5(Histone Deacetylase 5) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs)andhistone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Like HDAC4, HDAC5 shuttles between the nucleus and cytoplasm. In muscle cells, this protein shuttles into the cytoplasm during myocyte differentiation. HDAC5 interacts with many different transcription factors including HDAC3, and may form multi-complex proteins. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 120 kDa |
| 参考文献 |
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HDAC5 antibody tested by Western blot. Detection of HDAC5 by Western blot. NIH/3T3 cell extract was blotted and probed with HDAC5 antibody at a 2 µg/ml dilution. |
