| 货号 | 39933 |
| 别名 | WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µl per IP WB: 1:500 - 1:2,000 dilution Published Applications: WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2. |
| 背景 | EZH2–(Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 96 kDa |
| 参考文献 |
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EZH2 antibody (pAb) tested by Immunoprecipitation. 10 µl of EZH2 antibody was used to immunoprecipitate EZH2 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the EZH2 antibody at a dilution of 1:500. | |
EZH2 antibody tested by Western blot. Detection of EZH2 by Western blot. The analysis was performed using 20 µg HeLa acid extract and EZH2 antibody at a 1:500 dilution. |
