| 货号 | 39911 |
| 别名 | SIR2L6 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µl per IP WB: 1:500 - 1:2,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This SIRT6 antibody was raised against a peptide within the C-terminus of human SIRT6. |
| 背景 | SIRT6(Sir2 homologue 6) is an NAD+ dependant histone deacetylase (HDAC) related to SIRT1 and to the yeast Sir2 protein. SIRT6, originally identified as a tubulin deacetylase, it was recently found to be able to deacetylate histone H3 acetylated at lysine 9. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 46 kDa |
| 参考文献 |
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SIRT6 antibody (pAb) tested by Immunoprecipitation. 10 µl of SIRT6 antibody was used to immunoprecipitate SIRT6 from 500 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SIRT6 antibody at a dilution of 1:1,000. | |
SIRT6 antibody (pAb) tested by Western blot. HeLa cells nuclear extract (25 μg) probed with SIRT6 antibody (1:1,000). |
