| 货号 | 39903 |
| 别名 | SIR2, SIR2L, SIR2L2 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µl per IP WB: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This SIRT2 antibody was raised against a synthetic peptide derived from the C-terminal region of human SIRT2. |
| 背景 | SIRT2 is a member of the SIR2 / Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARγ and subsequent repression of PPARγ mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 40 & 45 kDa |
| 参考文献 |
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SIRT2 antibody (pAb) tested by Immunoprecipitation. 10 µl of SIRT2 antibody was used to immunoprecipitate SIRT2 from 250 µg of K-562 cytoplasmic cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SIRT2 antibody at a dilution of 1:500. | |
SIRT2 antibody (pAb) tested by Western blot. Cytoplasmic extract of K-562 cells (25 µg per lane) probed with SIRT2 antibody (1:500). |
