| 货号 | 39869 |
| 别名 | VT, VT4 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Ubinuclein antibody was raised against a recombinant protein corresponding to amino acids 1-345 of human Ubn1. |
| 背景 | UBN1(Ubinuclein 1, also known as HIRA binding protein) is a ubiquitously expressed nuclear protein that acts as a novel regulator of senescence. UBN1 is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. It is expressed in a number of cancer cell lines and tumors and may be required for replication-independent chromatin assembly. UBN1 is part of a large multi-subunit protein complex that interacts with CEBPA and Epstein-Barr virus BLZF1. It co-localizes with HIRA, PML and SP100 in the PML bodies of senescent cells. When overexpressed, UBN1 accumulates in the nucleus of cells defective for cytokinesis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 145 kDa |
| 参考文献 |
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Ubn1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 μg) probed with Ubn1 antibody at a dilution of 1:500. |
