| 货号 | 39721 |
| 别名 | Acf, ACF, Acf-1, ACF/CHRAC, ACF1, CAF, CG1966, CHRAC, CHRAC-175, dACF, dCHRAC, Dmel\CG1966, p170/p185 |
| 同种亚型 | IgG |
| 反应种属 | Drosophila |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:500 - 1:1,000 dilution WB: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Acf1 antibody was raised against recombinant C-terminal fragment of Acf1 from Drosophila melanogaster. |
| 背景 | DrosophilaAcf1 is a component of ACF, the ATP-utilizing chromatin assembly and remodeling factor protein complex. ACF catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and ISWI. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol. Sodium azide is highly toxic. |
| 计算分子量 | 185 kDa |
| 参考文献 |
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Acf1 pAb tested by immunofluorescence. Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution). | |
Acf1 pAb tested by Western blot. Drosophila Schneider L2 cells nuclear extract (10 µg) probed with Acf1 pAb (1:500 dilution). |
