| 货号 | 39675 |
| 别名 | RELA, p65, NFKB3 |
| 同种亚型 | Serum |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This NFkB p65 phospho Ser536 antibody was raised against a peptide containing phospho-serine 536 of human NFkB p65. |
| 背景 | NFκBp65(Nuclear factorNF-kappa-B p65subunit also known as NFκB p65orRelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1)and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB. NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA. Phosphorylation at serine 536 stimulates acetylation at lysine 310 and interaction with the acetyltransferase CBP. The phosphorylated and acetylated forms show enhanced transcriptional activity. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 65 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
NFκB p65 phospho Ser536 pAb tested by Western blot. HeLa cell extract (20 µg per lane) probed with either NFκB p65 phospho Ser536 pAb (Lanes 1 and 2, 1:500 dilution) or with NFκB p65 pAb (Catalog No. 39369, Lanes 3 and 4, 1:5,000 dilution). Lanes 1 and 3: extracts derived from untreated cells. Lanes 2 and 4: extracts derived from cells treated with TNF-α and Calyculin. | |
NFκB p65 phospho Ser536 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of of NFκB p65 phospho Ser536 pAb for NFκB p65 phosphorylated at serine 536. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with NFκB p65 phospho Ser536 pAb at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: phospho Ser276 NFκB p65 peptide. Lane 2: unmodified peptide surrounding Ser276 NFκB p65 peptide. Lane 3: phospho Ser529 NFκB p65 peptide. Lane 4: unmodified peptide surrounding Ser529 NFκB p65. Lane 5: phospho Ser536 NFκB p65 peptide. Lane 6: unmodified peptide surrounding Ser536 NFκB p65. Lane 7: phospho Ser337 NFκB p50 peptide. Lane 8: unmodified peptide surrounding Ser337 NFκB p50. |
