| 货号 | 39579 |
| 别名 | CG8677 |
| 同种亚型 | IgG |
| 反应种属 | Drosophila |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:1,000 - 1:5,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Rsf1 antibody was raised against a His-tagged fusion protein corresponding to amino acids 165-350 of DrosophilaRsf1. |
| 背景 | Rsf-1 (Remodeling and spacing factor 1) is a member of RSF complex. This complex consists of two subunits: Rsf-1 and SNF2H. RSF possesses a chromatin remodeling activity and a chromatin assembly activity. Rsf-1 assembles the nucleosomes randomly with its histone chaperone activity. The resulting nucleosomes are then redistributed by the ATP-utilizing nucleosome mobilization factor SNF2H into a periodic array. Rsf-1 can be involved in transcriptional repression when it is recruited to DNA via the DNA binding of Gal4 or in transcriptional coactivation when associated with hepatitis B virus pX protein (HBX). In response to hepatitis B infection, Rsf-1 can bind to the viral transcription activator pX, which then coactivates the transcription factor NFκB, thus supporting the life cycle of the viral genome. Additionally, Rsf-1 is thought to be a potent oncogene, with elevated expression observed in ovarian carcinomas. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol. Sodium azide is highly toxic. |
| 计算分子量 | 58 kDa |
| 参考文献 |
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Rsf1 pAb tested by Western blot. Schneiders Drosophila L2 nuclear extract (10 µg) was probed with Rsf1 pAb (1:1,000 dilution). |
