| 货号 | 39309 |
| 别名 | AP1, AP-1, JUN |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Electrophoretic Mobility Shift Assay |
| 使用方法 | Validated Applications: EMSA: 6 µl per assay Published Applications: see references The addition of Tris-boric electrophoresis buffer for supershift assays is recommended. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This c-Jun antibody was raised against a synthetic peptide corresponding to amino acid residues 20-37 of human c-Jun. |
| 背景 | c-Jun is a component of the AP-1 transcription factor complex in combination with c-Fos. It can be activated by phosphorylation from the JNK pathway. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic. |
| 计算分子量 | 41 kDa |
| 参考文献 |
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c-Jun antibody tested by supershift. Detection of c-Jun by EMSA analysis. c-Jun antibody was used in a supershift assay (EMSA) with 3T6 Swiss albino nuclear extract (lane 4). Lane 5 demonstrates a supershift competition in the presence of neutralizing peptide. |
