| 货号 | 39224 |
| 别名 | UBC13, UbcH13, HEL-S-71, UbcH-ben |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 2 µg/ml dilution For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This UBE2N antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 2-19 and 131-148 of human UBE2N. |
| 背景 | UBE2N(UBC13,BLU,ubiquitin-conjugating enzyme E2N) heterodimer with UBE2V2 catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBE2N mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UBE2N also plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage and acts together with the E3 ligase SHPRH in the poly-ubiquitination of PCNA Lys-164 upon genotoxic stress, which is required for DNA repair. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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UBE2N pAb tested by Western blot. UBE2N detection by Western blot. The analysis was performed using human heart whole-cell extract and UBE2N pAb at a 2 µg/ml dilution. |
