| 货号 | 39223 |
| 别名 | TERF2, TRBF2 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Goat |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 2 µg per IP WB: 1 - 2 µg/ml dilution For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This TRF2 antibody was raised against a full-length protein corresponding to human TRF2. |
| 背景 | TRF2 is a sequence-specific DNA binding protein that binds the duplex array of TTAGGG repeats at human telomeres. In particular, the telomere-binding protein TRF2 regulates both telomere protection and telomere length (end to end fusions are avoided through the formation of the t-loop). Telomere shortening is envisioned as a potent tumor suppressor mechanism. TRF2 is overexpressed in several human tumors, such as breast carcinomas, liver hepatocarcinomas, and lung carcinomas suggesting a general role in tumorigenesis. Removal of TRF2 results in the localization of active ATM to the unprotected telomeres, eventually inducing ATM-dependent apoptosis in telomerase-positive tumor cell lines. TRF2 has been shown to interact with ATM, through S1981 in an unphosphorylated state, and block its activation. It also interacts with several DNA repair proteins that are involved in human chromosomal instability syndromes characterized by premature aging and increased cancer. In particular, TRF2 interacts with Rap1, the WRN and BLM helicases, the DNA–PK complex, the MRE11/NBS1/Rad50 complex, PARP1 and PARP2 poly(ADP)ribosylases, and the ERCC1/XPF endonuclease. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 66 kDa |
| 参考文献 |
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TRF2 pAb tested by Western blot. Western Blot Analysis of TRF2 in HL-60 cells. Lane 1. IP with a mouse anti-TRF2. Lane 2. IP with control mouse IgG. Lane 3 IP with TRF2 pAb (Cat. No. 39223.) Lane 4. IP with pre-immune goat Ig. Lanes 1-4. WB with mouse anti-TRF2. TRF2 is detected as an ~ 66 kDa protein. |
