| 货号 | 39222 |
| 别名 | CCYC, R24L, RAD24, HRAD17, RAD17SP |
| 同种亚型 | IgG |
| 反应种属 | Human, Rat |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 0.1 - 1 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Rad17 phospho Ser647 antibody was raised against a synthetic peptide corresponding to mouse Rad17 phosphorylated at serine 647 . |
| 背景 | Rad17 Ser647(Rad24,CCYC,R24L) is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 has a weak ATPase activity required for binding to chromatin. Rad17 participates in the recruitment of the Rad1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. Rad17 may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 77 kDa |
| 参考文献 |
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Rad17 phospho Ser647 pAb tested by Western blot. Rad17 detection by Western blot. The analysis of phospho-RAD17 was performed using the Rad17 phospho Ser647 pAb and partial recombinant Rad17 protein containing phospho Ser647 (lane A) and the same sequence without a phospho serine (lane B). |
