货号 | 39200 |
别名 | APEX1, APX, APE1, APEN, APEX, HAP1, REF1 |
同种亚型 | IgG |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | Western Blot |
使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
供应商 | Active Motif |
纯化方式 | Affinity Purified |
免疫原 | This APE antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 36-52 and 216-232 of human APE. |
背景 | APE(apurinic endonuclease or apyrimidinic endonuclease) is a multifunctional DNA repair enzyme. The main role of APE is to create a nick in the phosphodiester backbone of the AP site that is created when DNA glycosylase removes the damaged base. Four types of APE (AP endonucleases) are classified according to their site of incision. |
运输条件 | Blue Ice |
存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
计算分子量 | 37 kDa |
参考文献 |
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APE pAb tested by Western blot. APE detection by Western blot. The analysis of APE was performed using HeLa whole-cell extract and APE pAb at a 2 µg/ml dilution. |