| 货号 | 39184 |
| 同种亚型 | Serum |
| 反应种属 | Other (Not Species Specific) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: IP: 1 µl/ 200 µg lysate WB: 1:25,000 - 1:100,000 dilution Published Applications: see references Validation for Western blot was confirmed using a LexA-Rb fusion and a LexA-PP2A, β subunit fusion expressed in budding yeast. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This LexA DNA-binding Domain antibody was raised against recombinant protein corresponding to full-length E. coliLexA. |
| 背景 | LexA is a prokaryotic protein that normally functions to repress the expression of DNA-damage response genes. The LexA DNA binding domain is frequently used as an epitope tag or fused to the "bait" protein as part of the yeast two-hybrid protein interaction system. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 参考文献 |
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LexA DNA-binding Domain pAb tested by Western blot. 20 µg of crude yeast protein extracts probed with LexA DNA-binding Domain pAb (1:100,000 dilution). Lane 1: Extract from wild-type yeast. Lane 2: Extract from yeast expressing a LexA-Retinoblastoma (Rb) fusion protein. |
