| 货号 | 39029 |
| 别名 | HMGB2 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution For optimal results, primary antibody incubations should be performed overnight at 37°C. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This HMG-2 antibody was raised against a synthetic peptide corresponding to amino acid residues 166-181 of human HMG-2. |
| 背景 | High mobility group proteins (HMG) are non-histone chromatin-associated proteins involved in DNA organization and regulation of transcription. The HMG protein family consists of six proteins and is subdivided into three subfamilies: the HMG-1/-2 subfamily, the HMG-I/Y subfamily and the HMG-14/-17 subfamily. Constitutively expressed in the nucleus, HMG-1, a class II HMG member, binds to bent or distorted DNA such as four-way DNA junctions, synthetic DNA cruciforms, kinked cisplatin-modified DNA, DNA bulges, cross-overs in supercoiled DNA. It can cause looping of linear DNA, thus functioning as an architectural element that facilitates interactions of neighboring proteins and DNA by creating favorable DNA conformations. The HMG-1 domain (often referred to as the HMG-1 box) is the functional motif of the largest HMG subfamily, the HMG-1 and HMG-2 proteins. These proteins change the conformation of DNA upon binding, but through a mechanism distinct from the more numerous histones. Extremely versatile HMG-1 and HMG-2 are involved in numerous DNA events in the nucleus such as DNA repair, replication, recombination and transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic. |
| 计算分子量 | 28 kDa |
| 参考文献 |
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HMG-2 pAb tested by Western blot. Detection of HMG-2 by Western blot analysis. HMG-2 is detected in nuclear extracts derived from the human K-562 cell line using HMG-2 rabbit polyclonal antibody. Lanes 1-3 represent serial dilutions of the antibody (1:500, 1:1,000 and 1:2,000 respectively). |
