| 货号 | 39617 |
| 别名 | MGF, STAT5 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This STAT5A/B phospho Tyr694/Tyr699 antibody was raised against a peptide containing phospho Tyr694 of human STAT5A. Human STAT5B shares the identical sequence surrounding Tyr699. |
| 背景 | STAT5A/B(signal transducer and activator of transcription 5) is a member of the STAT family of transcription factors. STATs undergo serine and tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT5 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNα, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others. Other members of the STAT family include STAT1α, STAT2, STAT3, STAT4, STAT5A, STAT5B and STAT6. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 92 kDa |
| 参考文献 |
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STAT5A/B phospho Tyr694/Tyr699 rabbit pAb tested by Western blot. Nb2 nuclear extract (20 µg per lane) was probed with STAT5A/B phospho Tyr694/Tyr699 rabbit pAb (1:2,000). Lane 1: No treatment. Lane 2: Cells treated with prolactin (25 ng/ml for 20 minutes at 37°C). | |
STAT5A/B phospho Tyr694/Tyr699 rabbit pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of STAT5A/B phospho Tyr694/Tyr699 rabbit pAb for STAT5A/B phospho Tyr694/699. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide. |
