| 货号 | 39037 |
| 别名 | p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution Published Applications: see references For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This NFkB p50 antibody was raised against a synthetic peptide corresponding to amino acid residues 363-376 of human NFκB p50. |
| 背景 | NFκB p50 is a member of the NFκB family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1) and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family. The NFκB p65/p50 heterodimer is the most abundant form of NFκB. NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic. |
| 计算分子量 | 50 kDa |
| 参考文献 |
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NFκB p50 antibody tested by Western blot. Detection of NFκB p50 by Western blot analysis. NFκB p50 can be detected in nuclear extracts derived from Raji cells using NFκB p50 rabbit polyclonal antibody. Lanes 2-4 demonstrate progressive titration of the antibody at 1:100, 1:500 and 1:1,000 dilutions, while lane 1 illustrates elimination of the signal in the presence of the immunizing peptide. |
