| 货号 | 61683 |
| 克隆类别 | 5A10 |
| 别名 | CHD-5, AW060752, 4930532L22Rik, B230399N07Rik |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Rat |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 2 - 5 µg/ml dilution WB: 1 - 2 µg/ml dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a peptide within the C-terminal region of mouse Chd5. |
| 背景 | Chd5 (Chromodomain Helicase DNA Binding Protein 5) is member of the CHD subfamily of chromatin remodeling SWI/SNF proteins, containing chromodomains and a SNF2-like ATPase/helicase domain, that play a role in chromatin remodeling and gene transcription. Chd5 is also a potential tumor suppressor that may play a role in neuronal development. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 260 kDa |
| 参考文献 |
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Chd5 antibody (mAb) (Clone 5A10) tested by immunofluorescence. Left: HeLa cells. Right: E12.5 mouse gut. Both stained with Chd5 antibody. | |
Chd5 antibody (mAb) (Clone 5A10) tested by Western blot. Chd5 antibody detection by Western blot. The analysis was performed using 40 µg of HeLa nuclear extract and Chd5 antibody at a 2 µg/ml dilution. |
