货号 | 61681 |
克隆类别 | 5F2G4 |
别名 | CAPH2 |
同种亚型 | IgG2a |
反应种属 | Human |
来源宿主 | Rat |
应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
使用方法 | Validated Applications: ICC/IF: 1 - 2 µg/ml dilution WB: 0.5 - 2 µg/ml dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
供应商 | Active Motif |
纯化方式 | Protein G Chromatography |
免疫原 | This antibody was raised against a recombinant protein to human NCAPH2. |
背景 | NCAPH2 (Non-SMC Condensin II Complex, Subunit H2) Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development. (By similarity) |
运输条件 | Blue Ice |
存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
计算分子量 | 90 kDa |
参考文献 |
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NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence. Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody. | |
NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot. NCAPH2 antibody detection by Western blot. The analysis was performed using 30 µg of HeLa nuclear extract and NCAPH2 antibody at a 1 µg/ml dilution. |