| 货号 | 61627-AM |
| 克隆类别 | 2E6E1 |
| 别名 | ENK, ecat4, 2410002E02Rik |
| 同种亚型 | IgG2a |
| 反应种属 | Mouse |
| 来源宿主 | Rat |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 2 - 10 µg/ml dilution WB: 1 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a peptide comprising amino acids 2-25 of mouse Nanog. |
| 背景 | Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5-TAAT[GT][GT]-3 or 5-[CG][GA][CG]C[GC]ATTAN[GC]-3 (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity). |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 45 kDa |
| 参考文献 |
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Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence. Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining. | |
Nanog antibody (mAb) (Clone 2E6E1) tested by Western blot. Nanog antibody detection by Western blot. The analysis was performed using 30 µg of mouse ES nuclear extract and Nanog antibody at a 2 µg/ml dilution. |
