| 货号 | 61147-AM |
| 克隆类别 | ICN 5B5 |
| 别名 | hN1, TAN1 |
| 同种亚型 | IgG2b |
| 反应种属 | Human, Rat |
| 来源宿主 | Rat |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Notch1 antibody was raised against a recombinant protein corresponding to the intracellular domain of human Notch1. |
| 背景 | Notch1 is a member of the Notch family of receptors, which make up a highly conserved cell signaling system. Notch1 functions as a ligand-activated transcription factor that directly transduces extracellular signals in the cell surface into changes in gene expression in the nucleus. Notch1 is membrane bound, serving as an extracellular receptor for the Delta and Serrate family of ligands. Upon activation, the intracellular domain is cleaved and rapidly translocates into the nucleus where it forms a transcriptional complex with the RBP-J DNA binding protein and activates gene expression via recruitment of co-activators of the MAML family. Notch receptors play a critical role in cell fate specification during development and participate in multiple biological processes. Notch signaling is required for undifferentiated embryonic stem cells (ESCs) to participate in the formation of all three embryonic germ layers. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 120 kDa |
| 参考文献 |
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Notch1 mAb (Clone ICN 5B5) tested by Western blot. HeLa whole-cell extract (20 µg/lane) probed with Notch1 mAb (Clone ICN 5B5) (2 µg/ml dilution). |
