| 货号 | 61095 |
| 克隆类别 | 6E5 |
| 别名 | Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2, ZEB2, ZFHX1B, HSPC082, SMADIP1 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunohistochemistry/Immunocytochemistry |
| 使用方法 | Validated Applications: WB: 1 - 4 µg/ml dilution Published Applications: IHC ICC/IF WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This SIP1 antibody was raised against a recombinant protein corresponding to amino acids 1-360 of human SIP1. |
| 背景 | SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR). |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 180 kDa |
| 参考文献 |
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Western Blot: Nuclear extract of Saos-2 cells (20 µg per lane) probed with the SIP1 antibody (mAb) at a dilution of 1 µg/ml. |
