| 货号 | 40962 |
| 克隆类别 | 70B1413 |
| 别名 | ATF6A |
| 同种亚型 | IgG1k |
| 反应种属 | Hamster, Human, Mouse, Rat |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunohistochemistry/Flow Cytometry |
| 使用方法 | Validated Applications: FACS: 1 µg/1x106cells IHC: 1:10 - 1:500 dilution WB: 1 - 5 µg/ml dilution Published Applications: see references For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. In Western blots the binding pattern of ATF-6 may vary as the antibody may recognize both full-length and cleaved forms of ATF-6. Researchers should consult literature for reported band sizes in their sample or treatment conditions. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This ATF-6 antibody was raised against a partial protein containing amino acid residues 1-273 of human ATF-6. |
| 背景 | ATF-6(activating transcription factor 6) is a transmembrane transcription factor that acts during endoplasmic reticulum (ER) stress to activate transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.05% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 90 kDa |
| 参考文献 |
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ATF-6 antibody (mAb) tested by Flow Cytometry.Intracellular flow cytometric staining of 1x106 MCF-7 cells using ATF6 antibody (dark blue) at a dilution of 1 µg/1x106 cells. Isotype control shown in orange. | |
ATF-6 antibody (mAb) tested by Immunohistochemistry.ATF6 antibody was used in IHC to stain formalin-fixed, paraffin-embedded human placenta at a 10 µg/ml dilution. |
