| 货号 | 40929 |
| 克隆类别 | 25B204.1 |
| 别名 | CASP7 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Caspase-7 antibody was raised against a recombinant human Caspase-7 protein. |
| 背景 | Caspase-7(CASP7orapoptosis-related cysteine peptidase 7) is involved in the activation cascade of caspases responsible for apoptosis execution. The precursor form of Caspase-7 is cleaved by Caspase-3 and Caspase-10, which is activated by cell death stimuli and induces apoptosis. Caspase-7 cleaves and activates sterol regulatory element binding proteins (SREBPs) and proteolytically cleaves poly(ADP-ribose) polymerase (PARP, see PARP-1 C-terminal and PARP-1 N-terminal antibodies). |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 35 kDa |
| 参考文献 |
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Caspase-7 mAb tested by Western blot. Caspase-7 detection by Western blot. The analysis was performed using Jurkat nuclear extracts and Caspase-7 mAb at dilutions of 2 µg/ml (lane 1) and 0.5 µg/ml (lane 2). This antibody only detects the 35 kDa caspase-7 corresponding to pro-caspase-7 in non-apoptotic Jurkat cells. |
