| 货号 | 40924 |
| 克隆类别 | 31A1067 |
| 别名 | CASP3 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 2 - 4 µg/ml dilution Published Applications: see references For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Caspase-3 antibody was raised against a 6xHis-tagged, full-length human Caspase-3 protein. |
| 背景 | Caspase-3(CASP3orapoptosis-related cysteine peptidase 3) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-3 cleaves and activates Caspase-6, Caspase-7 and Caspase-9, and the protein itself is processed by Caspase-8, Caspase-9 and Caspase-10. Caspase-3 is the predominate caspase involved in the cleavage of amyloid-beta 4A, which is associated with Alzheimers disease. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 35, 12, and 17 kDa |
| 参考文献 |
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Caspase-3 mAb tested by Western blot. Western blot analysis for detection of Caspase-3 activation. HeLa cells were treated with 2 µM staurosporine for different time periods and then probed using Caspase-3 mAb. Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively. |
