| 货号 | 40916 |
| 克隆类别 | 112A1021 |
| 别名 | RELA, p65, NFKB3 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 2 - 5 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This NFkB p65 antibody was raised against a synthetic peptide corresponding to amino acid residues 526-539 of human NFκB p65. |
| 背景 | NFκBp65(Nuclear factorNF-kappa-B p65subunit also known as NFκB p65orRelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1)and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB. NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 65 kDa |
| 参考文献 |
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NFκB p65 mAb tested by Western blot. NFκB p65 detection by Western blot. The analysis was performed using Daudi whole-cell extract and NFκB p65 mAb at a 2 µg/ml dilution. |
