| 货号 | 39877 |
| 克隆类别 | 2AO9 |
| 别名 | CHET9, JJAZ1 |
| 同种亚型 | IgG2b |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | ChIP/Immunoprecipitation/Immunohistochemistry |
| 使用方法 | Validated Applications: ChIP: 2.5 - 10 µg per ChIP Published Applications: IP IHC See references for more information. Individual optimization may be required. This antibody is not recommended for use in Western blotting. Use 39357instead. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Suz12 antibody was raised against a recombinant protein corresponding to full-length human Suz12. |
| 背景 | Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3 dimethyl Lys27 and Histone H3 trimethyl Lys27), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 95 kDa |
| 参考文献 |
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Suz12 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µg of Suz12 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the Myt1 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. |
