| 货号 | 39221 |
| 克隆类别 | 163C1251 |
| 别名 | PMSL2, HNPCC4, PMS2CL |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This PMS2 antibody was raised against a peptide corresponding to amino acid residues 623-639 of human PMS2. |
| 背景 | PMS2–Post-meiotic Segregation Increased 2 is the human homolog of the yeast Pms2 protein and is involved in post-replicative DNA mismatch repair (MMR). It forms a heterodimer with MLH-1 and this complex interacts with other protein complexes that recognize and bind to mismatched bases. PMS2 has endonuclease activity and introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation of the existing strand prevents cleavage and dictates that only the unmethylated, newly replicated (and likely to have the incorrect base) strand be repaired. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer and Turcot syndrome. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 85 kDa |
| 参考文献 |
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PMS2 mAb tested by Western blot. PMS2 detection by Western blot. The analysis was performed using NIH/3T3 whole-cell extract and PMS2 pAb at a 2 µg/ml dilution. |
